Abstract
Perforin and granzyme B are expressed primarily by activated
lymphocytes (cytotoxic T cells, natural killer cells, and natural killer T
cells) and function together to induce apoptosis of target cells. Typically,
these proteins are not expressed in tumor cells. In the present study, we
established the constitutive expression of perforin and granzyme B by the PC-3
and DU145 prostate cancer (PCA) cell lines with reverse transcription
polymerase chain reaction, immunohistochemistry, Western blot, or a combination
of techniques. The combination of radiation and resveratrol (XRT/RSV)
additively/synergistically decreased survival of PCA because, at least in part,
of increased apoptosis. We further demonstrated that treatment with RSV
up-regulated the expression of both perforin and granzyme B, whereas treatment
with XRT up-regulated the expression of granzyme B, but not that of perforin.
journal reference (abstract free): Journal of Andrology >>